The Screening Of Vaginal Lactobacilli

Screening of autochthonous vaginal beneficial lactobacilli

Screening of autochthonous vaginal beneficial lactobacilli strains by their growth at high temperatures for technological applications Antonie Van Leeuwenhoek 2020 Jul 28 doi: 101007/s10482020014314The pharmaceutical industry shows an emerging interest in formulas that contain live and beneficial microorganisms, also known as probiotics or pharmabiotics, which in many cases, are hostspecific The resistance to higher temperature is an essential feature of these microorganisms when working on the design of products for vaginal formula In order to obtain a high number of viable cellsScreening of autochthonous vaginal beneficial lactobacilli

Screening of biofilm formation by beneficial vaginal

Aims: To assess the ability of vaginal lactobacilli to form biofilm under different culture conditions and to determine the relationship between their growth and the capability of biofilm formation by selected strains Methods and results: Fifteen Lactobacillus strains from human vagina were tested for biofilm formation by crystal violet stainingDifferent human vaginal lactobacilli (VL) have been previously isolated, characterized and selected for their beneficial and technological properties by our research group (Ocaña et al 1999; Juárez Tomás et al 2003, 2005, 2011) Our longterm goal is the design of probiotics for vaginal application in women in order to prevent and/or treatScreening of biofilm formation by beneficial vaginal

Characterization of vaginal lactobacilli coaggregation

The coaggregation abilities of probiotic strains might enable it to form a barrier that prevents colonization by pathogenic bacteria In the present study, the characterization of the coaggregation ability of 19 vaginal lactobacilli was studied Coaggregation ability of all lactobacilli with EscheriWhile Gram staining of vaginal smears would offer an inexpensive and validated means for screening and diagnosis, probiotics that contain live lactobacilli capable of recolonizing the vagina may offer an elegant and safe choice of treatmentVaginal lactobacilli, probiotics, and IVF ScienceDirect

Vaginal lactobacilli, probiotics, and IVF

Perturbation of the normal lactobacillidominated vaginal microflora is associated with reproductive failure and adverse pregnancy outcomes, ranging from early pregnancy loss to late miscarriage and preterm birth As high rates of bacterial vaginosis are observed with IVF patients, abnormal vaginalAims: The isolation of bovine vaginal lactic acid bacteria (LAB) and the screening of their beneficial properties to select those that could be used as probiotics in the prevention of bovine metritis were performed Methods and Results: Out of 76 Lactobacillus sp and seven Streptococcus sp strains, a small number showed high and medium hydrophobicity when the microbial adhesion toProbiotic properties of vaginal lactic acid bacteria to

Contrasting diversity of vaginal lactobacilli among the

Background Lactobacilli are the predominant microbes in the vaginal microenvironment of healthy females []A key feature that favours colonization of Lactobacillus is low vaginal pH due to metabolism of glycogen to lactic acid; glycogen deposits in vaginal epithelial cells in response to rise in circulating estrogen [2, 3]The predominance of Lactobacillus promotes vaginal health byScreening for vaginal lactobacilli consensus sequences Consensus motifs in Lactobacillus genomes were sought by comparing selected full genome sequences using inhouse software developed on the VBA platform of MS Excel Briefly, the search algorithm scans a query sequence for all possible nucleotide motifs of 20 nucleotides or more andA novel realtime PCR assay for highly specific detection

Clinical Study of Vaginal Lactobacilli and Estriol

The purpose of this study is to assess the longterm safety and efficacy of the vaginal application of Gynoflor®, an extremely low dosed estrogen therapy with lactobacilli, on atrophic vaginitis in postmenopausal breast cancer patients who have been treated with aromatase inhibitorsThe healthy human vagina is dominated by lactobacilli, which play an important role in protecting the host from urogenital infections (4, 17, 18)Furthermore, it is widely recognized that the microbial balance between lactobacilli as the dominating flora and other, mainly gramnegative anaerobes can be upset and frequently result in the syndrome of bacterial vaginosis ()Vaginal Lactobacillus Flora of Healthy Swedish Women

Vaginal lactobacilli, probiotics, and IVF ScienceDirect

While Gram staining of vaginal smears would offer an inexpensive and validated means for screening and diagnosis, probiotics that contain live lactobacilli capable of recolonizing the vagina may offer an elegant and safe choice of treatmentThe vaginal microbiota of a healthy woman is often dominated by lactobacilli The most commonly identified Lactobacillus species include L crispatus, L gasseri, L jensenii and L iners [1–3]Lactobacilli are thought to play an important role in protecting the host from urogenital infection by lowering the environmental pH through lactic acid production, by producing various bacteriostaticIdentification and characterisation of vaginal

A novel realtime PCR assay for highly specific detection

Screening for vaginal lactobacilli consensus sequences Consensus motifs in Lactobacillus genomes were sought by comparing selected full genome sequences using inhouse software developed on the VBA platform of MS Excel Briefly, the search algorithm scans a query sequence for all possible nucleotide motifs of 20 nucleotides or more andThe protective potential of lactobacilli in pregnancy is based on their ability to inhibit the growth of vaginal microorganisms known to cause vaginal infection 10,11,26 Displacement of lactobacilli from the vagina frequently leads to an abnormal vaginal microflora, including BV, which, if present in early pregnancy, is a risk factor for PTDCharacterisation of the vaginal Lactobacillus microbiota

Contrasting diversity of vaginal lactobacilli among the

Background Lactobacilli are the predominant microbes in the vaginal microenvironment of healthy females []A key feature that favours colonization of Lactobacillus is low vaginal pH due to metabolism of glycogen to lactic acid; glycogen deposits in vaginal epithelial cells in response to rise in circulating estrogen [2, 3]The predominance of Lactobacillus promotes vaginal health byKeywords:Vaginal lactobacilli, beneficial properties, gastrointestinal tract resistance, viability, ascendant colonization, vaginal tract microbiome Abstract: Background: Lactobacilli are the dominant bacteria in the healthy vaginal tract, preventing the income of pathogenic microorganisms, either sexually or not transmittedSurvival of Beneficial Vaginal Lactobacilli (BVL) to

(PDF) Inhibitory Activity of Vaginal Lactobacilli Against

The receptors of the vaginal cells were glycolipids, which presumably were the targets of the competition observed between the lactobacilli and the pathogenic microbes View Show abstractThe aim of this study was to screen intestinal lactobacilli strains for their advantageous properties to select those that could be used for the development of novel gastrointestinal probiotics Ninetythree isolates were subjected to screening procedures Fiftynine percent of the examined lactobacilli showed the ability to autoaggregate, 97% tolerated a high concentration of bile (2% w/vScreening and Evaluation of Human Intestinal Lactobacilli

Vaginal Lactobacillus Flora of Healthy Swedish Women

The healthy human vagina is dominated by lactobacilli, which play an important role in protecting the host from urogenital infections (4, 17, 18)Furthermore, it is widely recognized that the microbial balance between lactobacilli as the dominating flora and other, mainly gramnegative anaerobes can be upset and frequently result in the syndrome of bacterial vaginosis ()The purpose of this study is to assess the longterm safety and efficacy of the vaginal application of Gynoflor®, an extremely low dosed estrogen therapy with lactobacilli, on atrophic vaginitis in postmenopausal breast cancer patients who have been treated with aromatase inhibitorsClinical Study of Vaginal Lactobacilli and Estriol

Role of Lactobacillus Species in the Intermediate Vaginal

Vaginal followup smears of the 349 women with intermediate vaginal flora and a Nugent score of 4 at the initial screening Discussion The present study aimed to evaluate the role of vaginal Lactobacillus spp in women with intermediate vaginal flora, as very little information is available on this matter in the literature [ 8 ]The vaginal microbiota of a healthy woman is often dominated by lactobacilli The most commonly identified Lactobacillus species include L crispatus, L gasseri, L jensenii and L iners [1–3]Lactobacilli are thought to play an important role in protecting the host from urogenital infection by lowering the environmental pH through lactic acid production, by producing various bacteriostaticIdentification and characterisation of vaginal

A novel realtime PCR assay for highly specific detection

Screening for vaginal lactobacilli consensus sequences Consensus motifs in Lactobacillus genomes were sought by comparing selected full genome sequences using inhouse software developed on the VBA platform of MS Excel Briefly, the search algorithm scans a query sequence for all possible nucleotide motifs of 20 nucleotides or more andThe protective potential of lactobacilli in pregnancy is based on their ability to inhibit the growth of vaginal microorganisms known to cause vaginal infection 10,11,26 Displacement of lactobacilli from the vagina frequently leads to an abnormal vaginal microflora, including BV, which, if present in early pregnancy, is a risk factor for PTDCharacterisation of the vaginal Lactobacillus microbiota

Contrasting diversity of vaginal lactobacilli among the

Background Lactobacilli are the predominant microbes in the vaginal microenvironment of healthy females []A key feature that favours colonization of Lactobacillus is low vaginal pH due to metabolism of glycogen to lactic acid; glycogen deposits in vaginal epithelial cells in response to rise in circulating estrogen [2, 3]The predominance of Lactobacillus promotes vaginal health byKeywords:Vaginal lactobacilli, beneficial properties, gastrointestinal tract resistance, viability, ascendant colonization, vaginal tract microbiome Abstract: Background: Lactobacilli are the dominant bacteria in the healthy vaginal tract, preventing the income of pathogenic microorganisms, either sexually or not transmittedSurvival of Beneficial Vaginal Lactobacilli (BVL) to

(PDF) Inhibitory Activity of Vaginal Lactobacilli Against

The receptors of the vaginal cells were glycolipids, which presumably were the targets of the competition observed between the lactobacilli and the pathogenic microbes View Show abstractThe aim of this study was to screen intestinal lactobacilli strains for their advantageous properties to select those that could be used for the development of novel gastrointestinal probiotics Ninetythree isolates were subjected to screening procedures Fiftynine percent of the examined lactobacilli showed the ability to autoaggregate, 97% tolerated a high concentration of bile (2% w/vScreening and Evaluation of Human Intestinal Lactobacilli

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